Journal: Bioactive Materials
Article Title: PKM2 + apoptotic vesicle-mediated systemic senolytics ameliorate chronic periodontitis
doi: 10.1016/j.bioactmat.2025.06.041
Figure Lengend Snippet: CP decreases the number of circulating EVs in mice. ( a and b ) Nanoparticle tracking analysis (NTA) by Zetaview revealed the number of mouse circulating EVs (16,000g and 120,000g) was decreased. (n = 5) ( c ) The expression of PKM2 in MSC-apoVs and MSC-exosomes (Exos) (both from the mice) was evaluated by Western blotting. ( d ) The PKM2 expression of circulating vesicles (16,000g and 120,000g) was compared between healthy people and periodontitis patients by Western blotting. ( e ) The decreased expression of PKM2 in circulating vesicles of the CP mice was confirmed by Western blotting. ( f – h ) Representative nano flow cytometry and IF staining showed a reduction in PKM2 + circulating vesicles (16,000g and 120,000g) in the CP mice group. (n = 3) Scale bar, 5 μm ( i and j ) Nano flow cytometry showed Annexin V positive (AV + ) circulating vesicles (16,000g and 120,000g) in the mouse plasma. (n = 3) For two-group comparisons, significance was assessed by unpaired Student's t -test. Error bars show the mean ± SD. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001. ns, not significant.
Article Snippet: MSC-apoVs were diluted in filtered PBS to appropriate concentrations and then monitored by ZetaView (Particle Metrix, Germany) for average particle size and electric potential.
Techniques: Expressing, Western Blot, Flow Cytometry, Staining, Clinical Proteomics